SK injection samples were manufactured by Xi'an Shi Ji Sheng Kang Pharmaceutical industry (Xi'an, China). The samples were diluted by four times to inject. The reference standards aloe-emodin, rhein, emodin, chrysophanol, physcion and astragaloside IV were purchased from Chengdu Weikeqi Bio-technology (Sichuan, China). Gallic acid, chlorogenic acid, caffeic acid, ferulic acid, rosmarinic acid, ethyl ferulate, protocatechuic acid, protocatechu aldehyde, catechin, hydroxysafflor yellow A

All the medicinal materials were tested, and their quality met the relevant standards of the 2020 edition of the Chinese Pharmacopoeia. Standard reference compounds, including epiberberine (wkq20031807), coptisine (wkq20021306), palmatine (wkq20051201), berberine (wkq20032408), baicalin (wkq20010908), wogonoside (wkq20040108), baicalein (wkq20022608), wogonin (20030204), rhein (wkq20052209), aloe-emodin (wkq20032708), emodin (wkq20041308), chrysophanol (wkq20081705), and physcion (wkq20040101) were purchased from Weikeqi Biotechnology Co., Ltd (Chengdu, China). The purity of all standard products was higher than 98%. Chromatographic grade acetonitrile and formic acid were purchased from Thermo-Fisher (US).

and kaempferol (≥98% Lot no. wkq19011609), aloe-emodin (≥98% Lot no. wkq19040302), ellagic acid (≥98% Lot no. wkq19010702), oroxylin a (≥98% Lot no. wkq19022608), and formononetin (≥98% Lot no. wkq19013002) from Sichuan Weikeqi Pharmaceutical Technology Co., Ltd.